Ethidium bromide: Destruction and decontamination of solutions
نویسندگان
چکیده
منابع مشابه
Mechanism of Ethidium Bromide Fluorescence Enhancement on
The mechanism of the enhancement of the fluorescence of ethidium bromide on binding to double helical RNA and DNA has been investigated. From an examination of the effect of different solvents on the fluorescence lifetime, quenching of fluorescence by proton acceptors, and the substantial lengthening of lifetime observed upon deuteration of the amino protons, regardless of the medium, we conclu...
متن کاملEthidium bromide-resistant mutant of Bacillus subtilis.
An ethidium bromide-resistant mutant (EB8) derived from a Marburg strain of Bacillus subtilis was found to be conditionally resistant to 10 mug of ethidium bromide per ml. Expression of resistance is complete only during vegetative growth at incubation temperatures above 30 C in complex medium or minimal medium supplemented with Casamino Acids. Strain EB8 is cross-resistant to acriflavine and p...
متن کاملStrand breakage in solutions of DNA and ethidium bromide exposed to visible light.
Breaks are introduced into DNA strands when DNA solutions containing ethidium bromide (EB) are exposed to incandescent light. The nicking rate is sensitive to the concentration of EB and the light intensity. At short exposure times, this rate is limited by photon capture and formation of an intermediate capable of nicking DNA and zero-order nicking kinetics are observed. If the EB is pre-irradi...
متن کاملPhotophysical Processes of Ethidium Bromide in Micelles and Reverse Micelles
Photophysical processes of ethidium bromide (EB) in homogeneous solutions, micelles, and reverse micelles have been investigated. In acetonitrile, the fluorescence intensity and lifetime of EB are 6.3 ( 0.3 times those in water and 1.25 ( 0.1 times those in acetone. This is attributed to the weaker hydrogen-bond acceptor property of acetonitrile, compared to water and acetone. Addition of water...
متن کاملA novel and robust conditioning lesion induced by ethidium bromide.
Molecular and cellular mechanisms underlying the peripheral conditioning lesion remain unsolved. We show here that injection of a chemical demyelinating agent, ethidium bromide, into the sciatic nerve induces a similar set of regeneration-associated genes and promotes a 2.7-fold greater extent of sensory axon regeneration in the spinal cord than sciatic nerve crush. We found that more severe pe...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1987
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90419-2